The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials

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Standard

The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials. / Draxler, Agnes; Franzke, Bernhard; Cortolezis, Johannes T; Gillies, Nicola A; Unterberger, Sandra; Aschauer, Rudolf; Zöhrer, Patrick A; Bragagna, Laura; Kodnar, Julia; Strasser, Eva-Maria; Neubauer, Oliver; Sharma, Pankaja; Mitchell, Sarah M; Zeng, Nina; Ramzan, Farha; D’Souza, Randall F; Knowles, Scott; Roy, Nicole Clemence; Sjödin, Anders Mikael; Mitchell, Cameron J; Milan, Amber M; Wessner, Barbara; Cameron-Smith, David; Wagner, Karl-Heinz.

In: Nutrients, Vol. 13, No. 10, 3479, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Draxler, A, Franzke, B, Cortolezis, JT, Gillies, NA, Unterberger, S, Aschauer, R, Zöhrer, PA, Bragagna, L, Kodnar, J, Strasser, E-M, Neubauer, O, Sharma, P, Mitchell, SM, Zeng, N, Ramzan, F, D’Souza, RF, Knowles, S, Roy, NC, Sjödin, AM, Mitchell, CJ, Milan, AM, Wessner, B, Cameron-Smith, D & Wagner, K-H 2021, 'The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials', Nutrients, vol. 13, no. 10, 3479. https://doi.org/10.3390/nu13103479

APA

Draxler, A., Franzke, B., Cortolezis, J. T., Gillies, N. A., Unterberger, S., Aschauer, R., Zöhrer, P. A., Bragagna, L., Kodnar, J., Strasser, E-M., Neubauer, O., Sharma, P., Mitchell, S. M., Zeng, N., Ramzan, F., D’Souza, R. F., Knowles, S., Roy, N. C., Sjödin, A. M., ... Wagner, K-H. (2021). The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials. Nutrients, 13(10), [3479]. https://doi.org/10.3390/nu13103479

Vancouver

Draxler A, Franzke B, Cortolezis JT, Gillies NA, Unterberger S, Aschauer R et al. The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials. Nutrients. 2021;13(10). 3479. https://doi.org/10.3390/nu13103479

Author

Draxler, Agnes ; Franzke, Bernhard ; Cortolezis, Johannes T ; Gillies, Nicola A ; Unterberger, Sandra ; Aschauer, Rudolf ; Zöhrer, Patrick A ; Bragagna, Laura ; Kodnar, Julia ; Strasser, Eva-Maria ; Neubauer, Oliver ; Sharma, Pankaja ; Mitchell, Sarah M ; Zeng, Nina ; Ramzan, Farha ; D’Souza, Randall F ; Knowles, Scott ; Roy, Nicole Clemence ; Sjödin, Anders Mikael ; Mitchell, Cameron J ; Milan, Amber M ; Wessner, Barbara ; Cameron-Smith, David ; Wagner, Karl-Heinz. / The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials. In: Nutrients. 2021 ; Vol. 13, No. 10.

Bibtex

@article{96d6a961293a40efbd99583553520027,
title = "The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials",
abstract = "A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine - DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in communitydwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.",
keywords = "Faculty of Science, Protein intake, Elderly, DNA damage, Comet assay, Glutathione",
author = "Agnes Draxler and Bernhard Franzke and Cortolezis, {Johannes T} and Gillies, {Nicola A} and Sandra Unterberger and Rudolf Aschauer and Z{\"o}hrer, {Patrick A} and Laura Bragagna and Julia Kodnar and Eva-Maria Strasser and Oliver Neubauer and Pankaja Sharma and Mitchell, {Sarah M} and Nina Zeng and Farha Ramzan and D{\textquoteright}Souza, {Randall F} and Scott Knowles and Roy, {Nicole Clemence} and Sj{\"o}din, {Anders Mikael} and Mitchell, {Cameron J} and Milan, {Amber M} and Barbara Wessner and David Cameron-Smith and Karl-Heinz Wagner",
note = "CURIS 2021 NEXS 309",
year = "2021",
doi = "10.3390/nu13103479",
language = "English",
volume = "13",
journal = "Nutrients",
issn = "2072-6643",
publisher = "M D P I AG",
number = "10",

}

RIS

TY - JOUR

T1 - The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials

AU - Draxler, Agnes

AU - Franzke, Bernhard

AU - Cortolezis, Johannes T

AU - Gillies, Nicola A

AU - Unterberger, Sandra

AU - Aschauer, Rudolf

AU - Zöhrer, Patrick A

AU - Bragagna, Laura

AU - Kodnar, Julia

AU - Strasser, Eva-Maria

AU - Neubauer, Oliver

AU - Sharma, Pankaja

AU - Mitchell, Sarah M

AU - Zeng, Nina

AU - Ramzan, Farha

AU - D’Souza, Randall F

AU - Knowles, Scott

AU - Roy, Nicole Clemence

AU - Sjödin, Anders Mikael

AU - Mitchell, Cameron J

AU - Milan, Amber M

AU - Wessner, Barbara

AU - Cameron-Smith, David

AU - Wagner, Karl-Heinz

N1 - CURIS 2021 NEXS 309

PY - 2021

Y1 - 2021

N2 - A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine - DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in communitydwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.

AB - A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine - DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in communitydwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.

KW - Faculty of Science

KW - Protein intake

KW - Elderly

KW - DNA damage

KW - Comet assay

KW - Glutathione

U2 - 10.3390/nu13103479

DO - 10.3390/nu13103479

M3 - Journal article

C2 - 34684481

VL - 13

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 10

M1 - 3479

ER -

ID: 281217459