TY - JOUR

T1 - Suppression of TNF-α production by S-adenosylmethionine in human mononuclear leukocytes is not mediated by polyamines

AU - Yu, Jingling

AU - Sauter, Senja

AU - Parlesak, Alexandr

N1 - (Ekstern)

PY - 2006

Y1 - 2006

N2 - Endotoxin-induced cytokine production is an important mechanism in the development of several types of liver damage. Methionine, some of its precursors and metabolites were reported to have protective effects against such injury. The aim of this study was to investigate whether methionine, its precursors or metabolites [phosphatidylcholine, choline, betaine, S-adenosylmethionine (SAM)] have a modulating effect on tumor necrosis factor α (TNF-α) production by endotoxin-stimulated human mononuclear leukocytes and whether SAM-dependent polyamines (spermidine, spermine) are mediators of SAM-induced inhibition of TNF-α synthesis. Methionine and betaine had a moderate stimulatory effect on TNF-α production, whereas phosphatidylcholine (ID50 5.4 μM), SAM (ID50 131 μM), spermidine (ID50 4.5 μM) and spermine (ID50 3.9 μM) had a predominantly inhibitory effect. Putrescine did not alter TNF-α release. Inhibitors of polyamine synthesis that blocked either putrescine (difluoromethylornithine) or spermine (CGP48664A) production did not affect TNF-α synthesis. Endotoxin stimulation of leukocytes did not alter the intracellular levels of polyamines. In addition, supplementation with SAM did not change the intracellular concentration of either polyamine measured. We conclude that phosphatidylcholine-induced immunosuppression is not caused by methionine and polyamines are not involved in SAM-induced inhibition of TNF-α production. The limitation of TNF-α release by spermidine is specific and is not due to its conversion into spermine.

AB - Endotoxin-induced cytokine production is an important mechanism in the development of several types of liver damage. Methionine, some of its precursors and metabolites were reported to have protective effects against such injury. The aim of this study was to investigate whether methionine, its precursors or metabolites [phosphatidylcholine, choline, betaine, S-adenosylmethionine (SAM)] have a modulating effect on tumor necrosis factor α (TNF-α) production by endotoxin-stimulated human mononuclear leukocytes and whether SAM-dependent polyamines (spermidine, spermine) are mediators of SAM-induced inhibition of TNF-α synthesis. Methionine and betaine had a moderate stimulatory effect on TNF-α production, whereas phosphatidylcholine (ID50 5.4 μM), SAM (ID50 131 μM), spermidine (ID50 4.5 μM) and spermine (ID50 3.9 μM) had a predominantly inhibitory effect. Putrescine did not alter TNF-α release. Inhibitors of polyamine synthesis that blocked either putrescine (difluoromethylornithine) or spermine (CGP48664A) production did not affect TNF-α synthesis. Endotoxin stimulation of leukocytes did not alter the intracellular levels of polyamines. In addition, supplementation with SAM did not change the intracellular concentration of either polyamine measured. We conclude that phosphatidylcholine-induced immunosuppression is not caused by methionine and polyamines are not involved in SAM-induced inhibition of TNF-α production. The limitation of TNF-α release by spermidine is specific and is not due to its conversion into spermine.

KW - Faculty of Science

KW - Inflammation

KW - Lipopolysaccharide

KW - Methionine

KW - Putrescine

KW - Spermidine

KW - Spermine

U2 - 10.1515/BC.2006.202

DO - 10.1515/BC.2006.202

M3 - Journal article

VL - 387

SP - 1619

EP - 1627

JO - Biological Chemistry Hoppe-Seyler

JF - Biological Chemistry Hoppe-Seyler

SN - 1431-6730

IS - 12

ER -