Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts
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Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts. / Sato, Ryoichi; Kawashima, Rinya; Trinh, Mai Duy Luu; Nakano, Masahiro; Nagai, Takeharu; Masuda, Shinji.
In: Photosynthesis Research, Vol. 139, No. 1-3, 18.06.2018, p. 359-365.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts
AU - Sato, Ryoichi
AU - Kawashima, Rinya
AU - Trinh, Mai Duy Luu
AU - Nakano, Masahiro
AU - Nagai, Takeharu
AU - Masuda, Shinji
PY - 2018/6/18
Y1 - 2018/6/18
N2 - The proton motive force (PMF) across the chloroplast thylakoid membrane that is generated by electron transport during photosynthesis is the driving force for ATP synthesis in plants. The PMF mainly arises from the oxidation of water in photosystem II and from electron transfer within the cytochrome b 6 f complex. There are two electron transfer pathways related to PMF formation: linear electron flow and cyclic electron flow. Proton gradient regulation 5 (PGR5) is a major component of the cyclic electron flow pathway, and the Arabidopsis pgr5 mutant shows a substantial reduction in the PMF. How the PGR5-dependent cyclic electron flow contributes to ATP synthesis has not, however, been fully delineated. In this study, we monitored in vivo ATP levels in Arabidopsis chloroplasts in real time using a genetically encoded bioluminescence-based ATP indicator, Nano-lantern(ATP1). The increase in ATP in the chloroplast stroma of pgr5 leaves upon illumination with actinic light was significantly slower than in wild type, and the decrease in ATP levels when this illumination stopped was significantly faster in pgr5 leaves than in wild type. These results indicated that PGR5-dependent cyclic electron flow around photosystem I helps to sustain the rate of ATP synthesis, which is important for growth under fluctuating light conditions.
AB - The proton motive force (PMF) across the chloroplast thylakoid membrane that is generated by electron transport during photosynthesis is the driving force for ATP synthesis in plants. The PMF mainly arises from the oxidation of water in photosystem II and from electron transfer within the cytochrome b 6 f complex. There are two electron transfer pathways related to PMF formation: linear electron flow and cyclic electron flow. Proton gradient regulation 5 (PGR5) is a major component of the cyclic electron flow pathway, and the Arabidopsis pgr5 mutant shows a substantial reduction in the PMF. How the PGR5-dependent cyclic electron flow contributes to ATP synthesis has not, however, been fully delineated. In this study, we monitored in vivo ATP levels in Arabidopsis chloroplasts in real time using a genetically encoded bioluminescence-based ATP indicator, Nano-lantern(ATP1). The increase in ATP in the chloroplast stroma of pgr5 leaves upon illumination with actinic light was significantly slower than in wild type, and the decrease in ATP levels when this illumination stopped was significantly faster in pgr5 leaves than in wild type. These results indicated that PGR5-dependent cyclic electron flow around photosystem I helps to sustain the rate of ATP synthesis, which is important for growth under fluctuating light conditions.
KW - Faculty of Science
KW - Photosynthesis
KW - PGR5
KW - ATP indicator
KW - Nano-lantern
KW - cyclic electron flow
KW - Chloroplast
UR - http://dx.doi.org/10.1007/s11120-018-0533-9
U2 - 10.1007/s11120-018-0533-9
DO - 10.1007/s11120-018-0533-9
M3 - Journal article
VL - 139
SP - 359
EP - 365
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1-3
ER -
ID: 311345397