TY - JOUR

T1 - Measurement of skeletal muscle glucose uptake in mice in response to acute treadmill running

AU - Møller, Lisbeth Liliendal Valbjørn

AU - Raun, Steffen Henning

AU - Fritzen, Andreas Mæchel

AU - Sylow, Lykke

N1 - CURIS 2022 NEXS 248

PY - 2022

Y1 - 2022

N2 - Skeletal muscle contractions stimulate glucose uptake into the working muscles during exercise. Because this signaling pathway is independent of insulin, exercise constitutes an important alternative pathway to increase glucose uptake, also in insulin-resistant muscle. Therefore, much effort is being put into understanding the molecular regulation of exercise-stimulated glucose uptake by skeletal muscle. To delineate the causal molecular mechanisms whereby muscle contraction or exercise regulate glucose uptake, the investigation of genetically manipulated rodents is necessary. Presented here is a modified and optimized protocol assessing exercise-induced muscle glucose uptake in mice in response to acute treadmill running. Using this high-throughput protocol, running capacity can accurately and reproducibly be determined in mice, and basal- and exercise-stimulated skeletal muscle glucose uptake and intracellular signaling can precisely and dose-dependently be measured in awake mice in vivo without the need for catheterization and with minimal loss of blood.

AB - Skeletal muscle contractions stimulate glucose uptake into the working muscles during exercise. Because this signaling pathway is independent of insulin, exercise constitutes an important alternative pathway to increase glucose uptake, also in insulin-resistant muscle. Therefore, much effort is being put into understanding the molecular regulation of exercise-stimulated glucose uptake by skeletal muscle. To delineate the causal molecular mechanisms whereby muscle contraction or exercise regulate glucose uptake, the investigation of genetically manipulated rodents is necessary. Presented here is a modified and optimized protocol assessing exercise-induced muscle glucose uptake in mice in response to acute treadmill running. Using this high-throughput protocol, running capacity can accurately and reproducibly be determined in mice, and basal- and exercise-stimulated skeletal muscle glucose uptake and intracellular signaling can precisely and dose-dependently be measured in awake mice in vivo without the need for catheterization and with minimal loss of blood.

KW - Faculty of Science

KW - AMPK

KW - Exercise

KW - Glucose uptake

KW - Muscle

KW - Muscle contraction

U2 - 10.14440/jbm.2022.385

DO - 10.14440/jbm.2022.385

M3 - Journal article

C2 - 36404875

VL - 9

JO - Journal of Biological Methods

JF - Journal of Biological Methods

SN - 2326-9901

IS - 3

M1 - e162

ER -