Discovery of urinary biomarkers of seaweed intake using untargeted LC-MS metabolomics in a three-way cross-over human study
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Discovery of urinary biomarkers of seaweed intake using untargeted LC-MS metabolomics in a three-way cross-over human study. / Xi, Muyao; Dragsted, Lars Ove; Tullin, Mikkel; Ernst, Madeleine; Zaharudin, Nazikussabah; La Barbera, Giorgia.
In: Metabolites, Vol. 11, No. 1, 11, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Discovery of urinary biomarkers of seaweed intake using untargeted LC-MS metabolomics in a three-way cross-over human study
AU - Xi, Muyao
AU - Dragsted, Lars Ove
AU - Tullin, Mikkel
AU - Ernst, Madeleine
AU - Zaharudin, Nazikussabah
AU - La Barbera, Giorgia
N1 - CURIS 2021 NEXS 008
PY - 2021
Y1 - 2021
N2 - Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In this three-armed, randomized, cross-over study, an untargeted metabolomics approach was applied for discovering novel intake biomarkers. Twenty healthy participants (9 men and 11 women) were provided each of three test meals in a randomized order: 5 g of Laminaria digitate (LD), 5 g of Undaria pinnatifida (UP), or a control meal with energy-adjusted pea protein. Four urine samples and a 24 h pooled urine were collected along with blood samples at seven time-points. All samples were profiled by LC-ESI-QTOF-MS and the data were analyzed by univariate analysis and excretion kinetics to select putative intake biomarkers. In total, four intake biomarkers were selected from urine samples. They were identified as hydroxyl-dihydrocoumarin at Level III, loliolid glucuronide at level I, and isololiolid glucuronide at level II, while the last one remains unknown. Further identification and validation of these biomarkers by a cross-sectional study is essential to assess their specificity and robustness.
AB - Seaweeds are a marine source rich in potentially bioactive components, and therefore have attracted attention since the middle of the twentieth century. Accurate and objective assessment of the intake of seaweeds to study their health effects is hampered by a lack of validated intake biomarkers. In this three-armed, randomized, cross-over study, an untargeted metabolomics approach was applied for discovering novel intake biomarkers. Twenty healthy participants (9 men and 11 women) were provided each of three test meals in a randomized order: 5 g of Laminaria digitate (LD), 5 g of Undaria pinnatifida (UP), or a control meal with energy-adjusted pea protein. Four urine samples and a 24 h pooled urine were collected along with blood samples at seven time-points. All samples were profiled by LC-ESI-QTOF-MS and the data were analyzed by univariate analysis and excretion kinetics to select putative intake biomarkers. In total, four intake biomarkers were selected from urine samples. They were identified as hydroxyl-dihydrocoumarin at Level III, loliolid glucuronide at level I, and isololiolid glucuronide at level II, while the last one remains unknown. Further identification and validation of these biomarkers by a cross-sectional study is essential to assess their specificity and robustness.
KW - Faculty of Science
KW - Seaweed
KW - Biomarkers of intake
KW - Untargeted metabolomics
U2 - 10.3390/metabo11010011
DO - 10.3390/metabo11010011
M3 - Journal article
C2 - 33379223
VL - 11
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 1
M1 - 11
ER -
ID: 254472279